Because of their lives with the cell walls, plant cells are masters of controlling cell division orientation and tight regulation of cellular growth. These two processes jointly shape plant organs. Our team focuses on regulation of cell elongation in root cells of Arabidopsis thaliana. We have currently established a vertical-stage spinning disk microscope which together with the available palette of genetically encoded fluorescent sensors and fluorescent dyes enables visualization of cellular physiology in a high spatio-temporal resolution. In the first part of the project, the candidate will use this toolbox to analyze the dynamics of root cell growth and physiological processes in response to phytohormones and selected nutrients. The second part of the project will focus on molecular players that drive cell elongation and differentiation processes in root cells. We have recently obtained transcriptomic profiles of Arabidopsis root tips in which excessive cellular elongation was triggered by different pharmacological and genetical means. The candidate will analyze this dataset, select most promising genes for further analysis and perform reverse genetics approach to find proteins that are crucial for regulation of longitudinal expansion in root cells.

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